PROFIL KROMATOGRAFI LAPIS TIPIS SENYAWA FLAVONOID DENGAN BERBAGAI MACAM ELUEN PADA EKSTRAK ETANOL DAUN GALAM (Melaleuca cajuputi) TANAMAN KHAS KALIMANTAN
Thin Layer Chromatography Profile Of Flavonoid Compounds With Various Eluents In Ethanol Extract Of Galam Leaves (Melaleuca cajuputi), Specific Plant Of Kalimantan
DOI:
https://doi.org/10.52674/gmkw6m53Keywords:
Galam leaf extract, Eluent, Flavonoids, Thin layer chromatography profileAbstract
Galam plant (Melaleuca cajuputi) has active compounds of galam plant that have anticancer, anti-inflammatory, antibacterial, hepatoprotective, anthelmintic activities. This research aims to find out the thin layer chromatography profile of flavonoid compounds in the ethanol extract of galam leaves. This research is a quantitative research with a laboratory experimental research design by analyzing the thin layer chromatography (TLC) profile of flavonoid compounds with various types of eluents, namely: Eluent n-Hexane: Ethyl acetate (8:2), Eluent n-Hexane: Dichloromethane (8:2) and Ethyl acetate: Dichloromethane (9:1) with quercetin as a comparator. The best eluent is expressed from the Rf value of the compound that is close to the Rf value of quercetin. The result of KLT of flavonoid compound with ethyl acetate:n-Hexane (8:2) eluent, quercetin showed an Rf value of 0.46 and 1 spot that is close to the Rf value of quecetin, namely A2 (0.47). In eluent n-Hexane: Dichloromethane (8:2) shows Rf value of quercetin 0.36 and Rf A1 value: 0.32 and in eluent Ethyl acetate: Dichloromethane (9:1) Rf value of quercetin is 0.76 and there are no spots close to the Rf value of Quercetin. The conclusion of the best eluent in the thin layer chromatography profile of the flavonoid compound of galam leaf extract is ethyl acetate:n-Hexane (8:2).
References
Elisabeth Oriana Jawa La, Repining Tiyas Sawiji, A. N. (2020). Skrining Fitokimia Dan Analisis Kromatografi Lapis Tipis Ekstrak Etanol Kulit Buah Naga Merah. 03(January), 45–58
Hafizah, D. A. (2024). Thin Layer Chromatographic Separation of Amino Acids by Determining the Resistivity Factor Value: Pemisahan Kromatografi Lapis Tipis pada Asam Amino dengan Menentukan Nilai Faktor Retensi. Jurnal Kimia dan Rekayasa, 5(1), 1-7
Isnaini, Joharman, Ananda, A. N., Ridho, A. R., Makati, A. C. (2023). Uji Toksisitas Ekstrak Metanol Daun Galam Dengan Metode Bslt. In Lambung Mangkurat Medical Seminar (vol 4, No. 1, pp 177-182).
Kumalasari, & Andiarna. (2020a). Uji Fitokimia Ekstrak Etanol Daun Kemangi (Ocimum Basilicum L). Journal For Health Sciences, 4(1), 39.
Nuryadin, Y., Naid, T., Dahlia, A. A., & Dali, S. (2018). Kadar flavonoid total ekstrak etanol daun serai dapur dan daun alang-alang menggunakan spektrofotometri UV-VIS. Window of Health: Jurnal Kesehatan, 337- 345.
Puspitasari, A. D., & Prayogo, L. S. (2017). Perbandingan metode ekstraksi maserasi dan sokletasi terhadap kadar fenolik total ekstrak etanol daun kersen (Muntingia calabura). Jurnal Ilmiah Cendekia Eksakta, 1(2), 1–8.
Salmia, S. (2016). Analisis Kadar Flavonoid Total Ekstrak Kulit Batang Kedondong Bangkok (Spondias dulcis) dengan Metode Spektrofotometri UV-Vis [PhD Thesis]. Universitas Islam Negeri Alauddin Makassar
Satria, R., Hakim, A. R., & Darsono, P. V. (2022). Penetapan Kadar Flavonoid Total Dari Fraksi nHeksana Ekstrak Daun Gelinggang dengan Metode Spektrofotometri UV-Vis. Journal of Engineering, Technology, and Applied Science, 4(1), 33-46.
Wardhani, A. K., Ariessanty, R. R., Akhyar, O., Prasiska, E., (2018). Skrining Fitokimia, Aktivitas Antioksidan, Dan Kadar Total Fenol-Flavonoid Ekstrak Daun Dan Buah Tanaman Galam Rawa Gambut (Melaleuca Cajuputi Roxb). Jurnal Inovasi Pendidikan Sains, Vol. 9, No.2, 2018, 13
