PENETAPAN KADAR FENOLIK TOTAL DAN NILAI SPF (SUN PROTECTION FACTOR) FRAKSI n-HEKSAN DARI EKSTRAK ETANOL DAUN JENGKOL (Pithecellobium lobatum)

Abstract

UV radiation from the sun can damage the strength and health of the skin. Skin protection from UV radiation, one of which can be done by using sunscreen. Synthetic sunscreens are known to have several risks. Plant extracts containing phenolic compounds are known to serve as an alternative to synthetic ingredients in sunscreen preparations.  Different types of solvents can result in various concentrations and compositions of bioactive compounds. This study aims to determine the total phenolic content and SPF value of the n-hexane fraction of jengkol leaves (Pithecellobium lobatum). Extraction is carried out by the maceration method using 96% ethanol then fractionation using a separate funnel with n-hexane solvent. The total phenolic levels were determined by the Folin-Ciocelteu method at a maximum wavelength of 732.45 and the determination of SPF values in the wavelength range of 290-320 nm using UV-Vis spectrophotometry. The results showed a total phenolic level of 113.96 ± 2.35 mg/g EAG and an SPF value at a concentration of 140 ppm of 1.820 ± 0.039.